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產(chǎn)品名稱:Vitamin C in Plasma/Serum - HPLC

產(chǎn)品型號(hào):65065

產(chǎn)品報(bào)價(jià):

產(chǎn)品特點(diǎn):

英文名:VitaminCinPlasma/Serum-HPLC供應(yīng)商:祥生興業(yè)規(guī)格:100TestsVitaminCinPlasma/Serum-HPLCOrderno

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65065Vitamin C in Plasma/Serum - HPLC的詳細(xì)資料:

溫馨提示:僅用于科研,不可用于臨床治療

  • 英文名:

    Vitamin C in Plasma/Serum - HPLC

  • 供應(yīng)商:

    祥生興業(yè)

  • 規(guī)格:

    100 Tests

    Vitamin C in Plasma/Serum - HPLC


    Order no.: 65065
    100 Tests
    • Vitamin C

    Simplified sample preparation
    Enhanced column stability
    High stability of standards and controls

    DESCRIPTION

    Clinical relevance

    Vitamin C (ascorbic acid, ascorbate) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.

    In the case of vitamin C deficiency, procollagen chains are produced insufficiently and stable fibrils cannot be formed in the affected cells. Without functioning collagen, the blood vessels and tendons become fragile and the typical skin-related symptoms associated with scurvy start to develop. In addition to the collagen damages described above, further diseases caused by vitamin C deficiency include bleeding of uncertain origin, loss of teeth and overactive thyroid gland (hyperthyroidism). Vitamin C deficiency is also observed in dialysis patients, in chronic alcohol abuse and during pregnancy.

     

    Product advantages

    • High stability of processed samples and controls
    • Enhanced column stability
    • Reliable quantification with a stable internal standard

     

    This Chromsystems kit allows the reliable chromatographic determination of vitamin C in one isocratic HPLC run with UV detection. An efficient protein precipitation step removes interfering components and stabilises Vitamin C simultaneously. A stable internal standard is used for reliable quantification. Due to a special stabilisation of the prepared samples, it is also possible to reliably process and measure large sample batches.

     

     

    CHROMATOGRAM

    65065 HPLC kit vitamin C plasma serum - Chromatogramm EN

    TECHNICAL DATA

    Method of Analysis

    HPLC

    Number of Tests

    100

    Parameter

    Vitamin C

    Analysis Time

    5 min

    Limit of quantification

    0.4 mg/l

    Linearity

    0.4–100 mg/l

    Recovery

    97–103 %

    Intraassay

    CV ≤ 3.8 %

    Interassay

    CV ≤ 4.8 %

    Specimen

    Plasma/Serum

    Pre-analytic Treatment

    Fresh lithium heparin plasma is used for analysis. To minimise the oxidation of vitamin C the whole blood sample should be centrifuged immediately after collection and the plasma samples must then be transported deep frozen. Start sample preparation immediately after thawing. The stability for vitamin C in heparin plasma is 5 days at -20 °C. At room temperature this is only up to two hours. If whole blood is used for transportation, store at +2 to +8 °C , do not freeze.

    Sample Preparation

    • Pipette 100 μl sample/calibrator/control into a labelled light protected reaction vial.

    • Add 100 μl Internal Standard and mix 30 s (vortex).

    • Centrifuge 5 min at at least 13000 x g.

    • Inject 20 μl of the supernatant into the HPLC system.

    Sample Stability

    The patient samples are stable for 12 h at room temperatur, at +2 to +8 °C for at least 2 weeks. For longer storage (up to 6 weeks) keep samples below -18 °C.

    Additional Info

    Any isocratic HPLC system with UV detection is suitable.

     

    Injection Volume

    20 µl

    Flow rate

    1.5 ml/min

    Gradient

    Isocratic

    Column temperature

    ambient (~25 °C)

    Wavelength

    245 nm

    Please note

    The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

    KIT COMPONENTS

    Components available separately

    Mobile Phase


    Order no.: 65001

    DETAILS

    Mobile Phase, 10 x 1000ml


    Order no.: 65002

    DETAILS

    Internal Standard


    Order no.: 65044

    DETAILS

    Reaction vials


    Order no.: 33005

    DETAILS

    Vitamin C Plasma Calibration Standard


    Order no.: 65003

    DETAILS

    ACCESSORIES

    PEEK prefilter housing


    Order no.: 15010

    DETAILS

    HPLC column


    Order no.: 65100

    DETAILS

    PEEK-encased prefilter, 5 µm


    Order no.: 15009

    DETAILS

    Precolumn cartridge 4/10


    Order no.: 18065

    DETAILS

    Precolumn cartridge holder 4/10


    Order no.: 18001

    DETAILS

    CALIBRATORS

    Calibrators available separately

    Vitamin C Plasma Calibration Standard


    Order no.: 65003

    DETAILS

    CONTROLS

    Controls available separately

    Vitamin C Plasma Controls


     

    DETAILS

溫馨提示:不可用于臨床治療。
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