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產品名稱:Vitamin C in Plasma/Serum - Automated HPLC

產品型號:65765/F

產品報價:

產品特點:

英文名:VitaminCinPlasma/Serum-AutomatedHPLC供應商:祥生興業(yè)規(guī)格:480TestsVitaminCinPlasma/Serum-AutomatedHPLCOrderno

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65765/FVitamin C in Plasma/Serum - Automated HPLC的詳細資料:

溫馨提示:僅用于科研,不可用于臨床治療

  • 英文名:

    Vitamin C in Plasma/Serum - Automated HPLC

  • 供應商:

    祥生興業(yè)

  • 規(guī)格:

    480 Tests

    Vitamin C in Plasma/Serum - Automated HPLC

    Order no.: 65765/F
    480 Tests
    • Vitamin C

    Automated sample preparation with 96 well filter plates

    DESCRIPTION

    Clinical relevance

    Vitamin C (ascorbic acid, ascorbate) is a water-soluble vitamin that is an essential cofactor for the human body, for instance in the catabolism of tyrosine and the synthesis of catecholamines. Regular consumption of fruit and vegetables is usually sufficient to cover the body’s vitamin C needs.

    In the case of vitamin C deficiency, procollagen chains are produced insufficiently and stable fibrils cannot be formed in the affected cells. Without functioning collagen, the blood vessels and tendons become fragile and the typical skin-related symptoms associated with scurvy start to develop. In addition to the collagen damages described above, further diseases caused by vitamin C deficiency include bleeding of uncertain origin, loss of teeth and overactive thyroid gland (hyperthyroidism). Vitamin C deficiency is also observed in dialysis patients, in chronic alcohol abuse and during pregnancy.

     

    Product advantages

    • High stability of processed samples and controls
    • Enhanced column stability
    • Reliable quantification with a stable internal standard

     

    This Chromsystems kit allows the reliable chromatographic determination of vitamin C in one isocratic HPLC run with UV detection. An efficient protein precipitation step removes interfering components and stabilises Vitamin C simultaneously. A stable internal standard is used for reliable quantification. Due to a special stabilisation of the prepared samples, it is also possible to reliably process and measure large sample batches.

     

    Kit content

    Mobile Phase, 1000 ml
    Vitamin C Plasma Calibration Standard (lyoph.), 5 x 0.5 ml
    Internal Standard, 10 ml
    96 Well filter plates, 5 pcs.
    Collection plates, 5 pcs.
    Pierceable adhesive seals for 96 well plates, 5 pcs.
     

     

    CHROMATOGRAM

    65765 F HPLC kit vitamin C - Chromatogram EN

    TECHNICAL DATA

    Method of Analysis

    HPLC

    Number of Tests

    480

    Parameter

    Vitamin C

    Analysis Time

    5 min

    Limit of quantification

    0.5 mg/l

    Linearity

    up to 120 mg/l

    Intraassay

    CV <= 3 %

    Interassay

    CV < 6 %

    Information

    Any isocratic HPLC system with UV detection is suitable.

    Specimen

    Plasma/Serum

    Pre-analytic Treatment

    Specimens: Fresh lithium heparin plasma

    Sample stability: 5 days at -20 °C, up to 2 hours at room temperature
    Detailed information on stability and transportation can be obtained from the instruction manual.

    Sample Preparation

    • Place 100 µl Internal Standard in a 96 well filter plate.
    • Add 100 µl sample/calibrator/control.
    • Vortex for 30 s at 1100 rpm (orbit: 2 mm).
    • Centrifuge for 5 min at 2000 x g.
    • Close the 96 well filter plate.
    • Vortex for 30 s at 800 rpm (orbit: 2 mm).
    • Inject 20 µl of the prepared sample into the HPLC system.

    Additional Info

    For the Chromsystems HPLC analysis of vitamin C any isocratic HPLC system with UV detection is suitable.

    Injection Volume

    20 µl

    Flow rate

    1.5 ml/min

    Flow

    1.5 ml/min

    Gradient

    Isocratic

    Column temperature

    ambient (~25 °C)

    Wavelength

    245 nm

    Please note

    The freely available information on this website, in particular on the sample preparation, are not sufficient to work with our products. Please read instructions and warning notices on products and/or instruction manuals.

    KIT COMPONENTS

    Components available separately

    Mobile Phase


    Order no.: 65001

    DETAILS

    Mobile Phase, 10 x 1000ml


    Order no.: 65002

    DETAILS

    Vitamin C Plasma Calibration Standard


    Order no.: 65003

    DETAILS

    Internal Standard


    Order no.: 65044

    DETAILS

    96 Well Filter Plates


    Order no.: 65757

    DETAILS

    Collection Plates


    Order no.: 65758

    DETAILS

    Pierceable adhesive seals for 96 Well Plates


    Order no.: 65759

    DETAILS

    ACCESSORIES

    PEEK prefilter housing


    Order no.: 15010

    DETAILS

    HPLC column


    Order no.: 65100

    DETAILS

    PEEK-encased prefilter, 5 µm


    Order no.: 15009

    DETAILS

    Precolumn cartridge 4/10


    Order no.: 18065

    DETAILS

    Precolumn cartridge holder 4/10


    Order no.: 18001

    DETAILS

    CALIBRATORS

    Calibrators available separately

    Vitamin C Plasma Calibration Standard


    Order no.: 65003

    DETAILS

    CONTROLS

    Controls available separately

    Vitamin C Plasma Controls


     

    DETAILS

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